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Updated: Oct 5, 2025
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In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model
Published on: January 21, 2018
9.4K
Quantification of Angiogenesis in Laser Choroidal Neovascularization
Kathryn R Green1, Nicholas Beazley-Long1, Amy P Lynch1
1Endothelial Quiescence Group, Centre for Cancer Sciences, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham, UK.
Methods in Molecular Biology (Clifton, N.J.)
|January 31, 2022
Summary
This study details a 14-day murine model of wet age-related macular degeneration (wAMD) using laser-induced choroidal neovascularization. This robust model aids in understanding wAMD and developing new treatments.
Area of Science:
- Ophthalmology
- Vascular Biology
- Regenerative Medicine
Background:
- Wet age-related macular degeneration (wAMD) is a leading cause of vision loss.
- Laser-induced choroidal neovascularization in mice is a key model for studying wAMD.
- Damage to Bruch's membrane causes fluid accumulation and vision impairment.
Purpose of the Study:
- To describe a 14-day murine model of laser-induced choroidal neovascularization.
- To establish a robust model for wAMD research.
- To facilitate therapeutic agent development for wAMD.
Main Methods:
- Utilizing a focused laser to induce damage to Bruch's membrane in C57/Bl6 mice.
- Establishing a 14-day experimental timeline for observation.
- Employing immunohistochemical staining for detailed analysis.
Main Results:
- A reproducible model of choroidal neovascularization was established.
- The model allows for in vivo functional analysis of lesioned choroids.
- The model is suitable for various therapeutic interventions and study durations.
Conclusions:
- The described murine model is a valuable tool for wAMD research.
- This model supports the development and testing of novel wAMD therapies.
- The model's versatility aids in advancing our understanding of retinal diseases.
