Related Experiment Video
Updated: Oct 5, 2025
08:37
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
5.5K
gga-miRNOME, a microRNA-sequencing dataset from chick embryonic tissues
Isabel Duarte1,2, Gil Carraco1,3,4, Nayara T D de Azevedo5
1Faculdade de Medicina e Ciências Biomédicas (FMCB), Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
Scientific Data
|February 1, 2022
Summary
This study characterizes microRNAs (miRNAs) in early chick embryo development, identifying 926 known and 1,141 novel miRNAs. These findings expand the Gallus gallus miRNA database and offer new insights into vertebrate embryogenesis.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- MicroRNAs (miRNAs) are crucial regulators of gene expression, particularly in early vertebrate development.
- A lack of miRNA datasets from non-mammalian embryos, like the chicken (Gallus gallus), limits research in comparative embryogenesis.
- The chicken embryo is a classical model for studying vertebrate development.
Purpose of the Study:
- To characterize microRNA profiles during early trunk and limb development in the chick embryo.
- To expand the publicly available miRNA dataset for Gallus gallus.
- To identify novel miRNAs potentially involved in vertebrate embryogenesis.
Main Methods:
- MicroRNA-sequencing (miRNA-seq) was performed on three distinct chick embryonic tissues.
- Tissues profiled included Undetermined Presomitic Mesoderm (PSM_U), Determined Presomitic Mesoderm (PSM_D), and Forelimb Distal Cyclic Domain (DCD).
Main Results:
- Identification of 926 known miRNAs.
- Discovery of 1,141 novel candidate miRNAs.
- The identified miRNAs nearly double the existing Gallus gallus entries in the miRBase database.
Conclusions:
- This study provides a comprehensive miRNA dataset for early chick embryo development.
- The findings significantly benefit the avian research community by offering new candidate miRNAs for further study.
- The identified miRNAs are potentially key regulators of early vertebrate embryo development and warrant experimental validation.
