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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Updated: Oct 5, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

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Editing of DNA Methylation Patterns Using CRISPR-Based Tools.

Jim Smith1, Rakesh Banerjee1, Robert J Weeks2

  • 1Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.

Methods in Molecular Biology (Clifton, N.J.)
|February 1, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a new CRISPR-based system for precise DNA methylation editing in mammalian cells. This technology overcomes limitations of older methods, enabling better understanding of epigenetic regulation in health and disease.

Keywords:
CRISPRDNAEditingMethylationTransfectiondCas9

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Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation is a key epigenetic modification influencing cellular function and disease.
  • Establishing a causal link between DNA methylation changes and gene expression has been challenging.
  • Previous methods for studying DNA methylation's role in gene regulation were limited by specificity, cost, and labor.

Purpose of the Study:

  • To develop a novel, site-specific DNA methylation editing system.
  • To overcome the limitations of existing epigenomic editing technologies.
  • To provide a detailed protocol for utilizing CRISPR-based epigenomic editing in mammalian cells.

Main Methods:

  • Design and construction of a transient CRISPR-based DNA methylation-editing system.
  • Application of the system in mammalian cell culture.
  • Protocol development for epigenomic editing.

Main Results:

  • Successful development of a CRISPR-based system for targeted DNA methylation editing.
  • Demonstration of the system's utility in mammalian cells.
  • A detailed protocol is provided for researchers.

Conclusions:

  • CRISPR-based technologies offer a revolutionary approach to epigenomic editing.
  • The developed system provides a powerful tool for investigating DNA methylation's role in gene regulation.
  • This advancement facilitates research into the causal relationships between DNA methylation and disease.