Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

578
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
578
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.2K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.2K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

324
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
324
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

335
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
335
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

1.7K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tracking the Early Hydration Reaction of Cementitious Calcium Silicate Hydrate via DNP-Enhanced Solid-State NMR.

Journal of the American Chemical Society·2026
Same author

Anatomical feasibility and experimental evaluation of sensor coupling sites for fully implantable active middle ear implants.

Hearing research·2026
Same author

Microbial and anthropogenic controls on global soil nitrogen mineralization efficiency.

Journal of environmental management·2026
Same author

Postcode-Level Variation and Environmental Determinants of Respiratory Allergies in a Large German Cohort.

Allergy·2026
Same author

Shortened olfactory identification testing to differentiate parkinsonian syndromes.

Parkinsonism & related disorders·2026
Same author

Clinical Relevance of Atrial Fibrillation in End-Stage Heart Failure Patients Actively Waiting on Heart Transplant.

Journal of cardiovascular development and disease·2026
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Oct 4, 2025

Hyperpolarized Xenon for NMR and MRI Applications
16:20

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

19.7K

Hyperpolarized Solution-State NMR Spectroscopy with Optically Polarized Crystals.

Tim R Eichhorn1, Anna J Parker1, Felix Josten1

  • 1NVision Imaging Technologies GmbH, 89081 Ulm, Germany.

Journal of the American Chemical Society
|February 3, 2022
PubMed
Summary
This summary is machine-generated.

Nuclear spin hyperpolarization enhances nuclear magnetic resonance (NMR) sensitivity. This study uses spin-polarized crystals to transfer polarization to target molecules, achieving signal enhancements of up to -1730x in just one minute.

More Related Videos

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.7K
Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K

Related Experiment Videos

Last Updated: Oct 4, 2025

Hyperpolarized Xenon for NMR and MRI Applications
16:20

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

19.7K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.7K
Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Quantum Information Science
  • Materials Science

Background:

  • Limited sensitivity of conventional NMR hinders its application in various scientific fields.
  • Nuclear spin hyperpolarization offers a pathway to significantly boost NMR signal intensity.
  • Efficient transfer of hyperpolarization to target molecules remains a key challenge.

Purpose of the Study:

  • To demonstrate a novel method for transferring hyperpolarization from optically polarized crystals to target molecules.
  • To achieve substantial NMR signal enhancement at room temperature and moderate magnetic fields.
  • To develop a practical protocol for utilizing hyperpolarized materials in benchtop NMR spectrometers.

Main Methods:

  • Dissolution of spin-polarized pentacene-doped naphthalene crystals.
  • Intermolecular cross-relaxation for polarization transfer to target molecules.
  • Injection of hyperpolarized mixture into a benchtop NMR spectrometer.
  • Data processing to mitigate radiation damping effects and extract polarization.

Main Results:

  • Successful transfer of hyperpolarization to target 1H nuclei at 1.45 T.
  • Observed NMR signal enhancements ranging from -200x to -1730x.
  • Demonstrated a complete process within a 1-minute time scale.
  • Developed a data processing technique to obtain conventional NMR spectra.

Conclusions:

  • The presented method effectively overcomes challenges in hyperpolarization transfer.
  • This technique significantly enhances NMR sensitivity for small molecules at room temperature.
  • The rapid and efficient process is compatible with benchtop NMR instrumentation, broadening accessibility.