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Long non-coding RNA SNHG3 promotes the progression of clear cell renal cell carcinoma via regulating BIRC5 expression

Zhaoyu Xu1, Junjie Ye1, Pengfei Bao1

  • 1Department of Urology, Lishui People's Hospital, Lishui, China.

Translational Cancer Research
|February 4, 2022
PubMed
Abstract

Insights

Long non-coding RNA SNHG3 promotes clear cell renal cell carcinoma (ccRCC) progression by interacting with microRNA-10b-5p and upregulating BIRC5. SNHG3 may be a novel therapeutic target for ccRCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Clear cell renal cell carcinoma (ccRCC) progression is influenced by long non-coding RNAs (lncRNAs).
  • The specific roles of lncRNAs in ccRCC malignancy remain largely unknown.

Purpose of the Study:

  • To investigate the role of novel lncRNAs in ccRCC progression.
  • To elucidate the molecular mechanisms underlying ccRCC malignancy involving lncRNAs and BIRC5.

Main Methods:

  • Utilized TCGA and GSE66272 datasets to identify differentially expressed genes in ccRCC.
  • Employed ENCORI database for miRNA network analysis and assessed lncRNA-miRNA interactions.
  • Performed quantitative real-time PCR, western blotting, and dual-luciferase reporter assays to validate findings and interactions.

Main Results:

  • Identified two novel lncRNAs, LINC00997 and SNHG3, upregulated in ccRCC tissues and positively correlated with BIRC5 protein expression.
  • Suppression of SNHG3 significantly reduced BIRC5 protein levels and inhibited tumor progression in vitro.
  • Demonstrated that SNHG3 promotes BIRC5 expression by sponging microRNA-10b-5p.

Conclusions:

  • SNHG3 plays a critical role in promoting ccRCC progression through the microRNA-10b-5p/BIRC5 axis.
  • SNHG3 represents a potential novel therapeutic target for ccRCC treatment.
Keywords:
BIRC5LncRNA SNHG3clear cell renal cell carcinoma (ccRCC)miR-10b-5p

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