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Knockdown of RPL34 suppresses osteosarcoma cell proliferation likely through EIF3/FAU signaling pathway

Piwei Huang1,2,3, Jinmin Zhao3,4, Mitra Fowdur1,3

  • 1Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530000, China.

Translational Cancer Research
|February 4, 2022
PubMed
Abstract

Insights

Knocking down ribosomal protein L34 (RPL34) inhibits osteosarcoma cell growth. This likely occurs by affecting EIF3A, EIF3F, and FAU gene expression, offering potential therapeutic targets.

Area of Science:

  • Molecular Biology
  • Cancer Research

Background:

  • Osteosarcoma is a primary bone cancer.
  • Ribosomal protein L34 (RPL34) role in osteosarcoma is unclear.
  • Eukaryotic initiation factor 3 (EIF3) and FAU are implicated in cancer progression.

Purpose of the Study:

  • To investigate the impact of RPL34 knockdown on osteosarcoma cell proliferation.
  • To explore the involvement of EIF3/FAU pathway in RPL34-mediated effects.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot analysis were used.
  • Gene expression of RPL34, EIF3A, EIF3F, EIF3G, UBA52, UBC, and FAU was measured.
  • RPL34 was knocked down using shRNA in Saos-2 osteosarcoma and hFOB1.19 osteoblast cell lines.

Main Results:

  • Saos-2 cells showed higher expression of RPL34, EIF3A, EIF3G, UBA52, UBC, and FAU compared to hFOB1.19 cells.
  • EIF3F expression was lower in Saos-2 cells.
  • RPL34 knockdown decreased EIF3A and EIF3F, and increased FAU expression in Saos-2 cells.

Conclusions:

  • RPL34 knockdown inhibits osteosarcoma cell proliferation.
  • The EIF3A, EIF3F, and FAU pathways are likely involved in this process.
Keywords:
EIF3FAURPL34osteosarcomasignaling pathway

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