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Triptonide inhibits metastasis potential of thyroid cancer cells via astrocyte elevated gene-1

Liangjie Fu1, Xiaohong Niu1, Ruhui Jin1

  • 1Department of Scrofulosis, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Nanjing 210014, China.

Translational Cancer Research
|February 4, 2022
PubMed
Abstract

Insights

Triptonide (TN) inhibits thyroid cancer metastasis by reducing astrocyte elevated gene (AEG-1) expression. This study reveals TN

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Triptonide (TN) has demonstrated anti-tumor properties.
  • The role of TN in thyroid cancer progression and its underlying mechanisms require elucidation.
  • Thyroid cancer metastasis is a significant clinical challenge.

Purpose of the Study:

  • To investigate the inhibitory effects of Triptonide (TN) on thyroid cancer.
  • To explore the molecular mechanisms by which TN affects thyroid cancer cell behavior.
  • To determine the role of astrocyte elevated gene (AEG-1) in TN's anti-cancer activity.

Main Methods:

  • Thyroid cancer cell lines (MDA-T68 and BCPAP) were treated with TN.
  • Cell viability, migration, and invasion were assessed using MTT and Transwell assays.
  • Protein and mRNA expression levels of key genes, including AEG-1, MMPs, and cadherins, were analyzed via Western blot and qPCR.

Main Results:

  • Triptonide (TN) significantly inhibited thyroid cancer cell viability, migration, and invasion at concentrations above 50 nmol/L.
  • TN treatment led to a decrease in astrocyte elevated gene (AEG-1) expression.
  • Overexpression of AEG-1 promoted cancer cell proliferation and metastasis, effects that were attenuated by TN, which also reversed AEG-1-induced changes in MMPs and cadherins.

Conclusions:

  • Triptonide (TN) effectively inhibits the metastatic potential of thyroid cancer cells.
  • The mechanism involves the downregulation of astrocyte elevated gene (AEG-1) expression.
  • These findings highlight TN as a potential therapeutic agent for thyroid cancer, warranting further investigation.

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