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Antibacterial effect of donor corneas stored in gentamicin-enriched McCarey-Kaufman medium
Abstract:
Penetrating keratoplasty was performed on five pairs of rabbit eyes; one eye of each animal received a donor cornea stored in McCarey-Kaufman (M-K) medium containing gentamicin, and the contralateral eye received a donor cornea stored in M-K medium without gentamicin. At the end of surgery, dilutions of Pseudomonas aeruginosa were introduced into the anterior chamber of both eyes. The eyes receiving gentamicin-enriched corneas showed mild to moderate infection; the contralateral eyes showed significantly more severe infection. The results indicated that donor corneas stored in gentamicin-enriched M-K medium inhibit the growth of bacteria acquired at the time of surgery and thus may improve the safety of such transplantation procedures.
Insights
Adding gentamicin to donor cornea storage medium significantly reduced infection after penetrating keratoplasty in rabbits. Gentamicin-enriched corneas offer enhanced protection against bacterial contamination during corneal transplantation.
Area of Science:
- Ophthalmology
- Microbiology
- Transplantation Science
Background:
- Penetrating keratoplasty is a common surgical procedure to restore vision.
- Bacterial contamination of donor corneas can lead to severe post-operative infections.
- McCarey-Kaufman (M-K) medium is a standard storage solution for donor corneas.
Purpose of the Study:
- To evaluate the efficacy of gentamicin in inhibiting bacterial growth in donor corneas.
- To assess the impact of gentamicin-enriched M-K medium on post-keratoplasty infection rates in a rabbit model.
Main Methods:
- Penetrating keratoplasty was performed on rabbit eyes using donor corneas stored in M-K medium with or without gentamicin.
- Pseudomonas aeruginosa was introduced into the anterior chamber post-surgery.
- Infection severity was assessed in both groups.
Main Results:
- Eyes receiving gentamicin-enriched corneas exhibited mild to moderate infections.
- Contralateral eyes (without gentamicin) showed significantly more severe infections.
- Gentamicin effectively inhibited bacterial growth acquired during surgery.
Conclusions:
- Donor corneas stored in gentamicin-enriched M-K medium demonstrate reduced post-operative infection.
- This method may enhance the safety and success rates of corneal transplantation procedures.
- Gentamicin incorporation into storage media is a promising strategy to prevent microbial contamination.