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miR-204-5p Hampers Breast Cancer Malignancy and Affects the Cell Cycle by Targeting PRR11

Qunxue Su1, Hao Shen1, Bei Gu2

  • 1Department of Pathology, The Second People's Hospital of Kunshan, Kunshan, 215300 Jiangsu, China.

Computational and Mathematical Methods in Medicine
|February 7, 2022
PubMed
Abstract

Insights

MicroRNA-204-5p (miR-204-5p) suppresses breast cancer (BC) progression by targeting PRR11. This discovery offers new insights into miR-204-5p

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Breast cancer (BC) remains a leading cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms underlying BC progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the role and regulatory mechanisms of microRNA-204-5p (miR-204-5p) in breast cancer (BC) cells.
  • To identify potential downstream targets of miR-204-5p involved in BC pathogenesis.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure miR-204-5p and PRR11 expression.
  • Bioinformatic prediction and dual-luciferase assays to validate miR-204-5p binding to PRR11.
  • Western blot analysis for protein expression.
  • Cell proliferation (CCK8), cell cycle (flow cytometry), and migration/invasion (Transwell) assays to assess cancer cell functions.

Main Results:

  • miR-204-5p was significantly downregulated in BC tissues and cell lines.
  • miR-204-5p suppressed BC cell proliferation, migration, invasion, and induced cell cycle arrest.
  • PRR11 was identified as a direct target of miR-204-5p.
  • Silencing PRR11 partially reversed the effects of miR-204-5p inhibition on BC cell functions.

Conclusions:

  • The miR-204-5p/PRR11 axis plays a suppressive role in breast cancer progression.
  • miR-204-5p acts as a tumor suppressor in BC by targeting PRR11.
  • This finding provides a novel therapeutic target and insight into BC regulation.

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