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Published on: March 30, 2018
Expression of apple MdMYB10 transcription factor in sugar beet with a screenable marker role and antimicrobial
Mitra Khademi1, Farhad Nazarian-Firouzabadi1, Ahmad Ismaili1
1Production Engineering and Plant Genetics Department, Faculty of Agriculture, Lorestan University, P.O. Box: 465, Khorramabad, Iran.
Abstract:
Selection of transgenic plants by using genes encoding screenable markers of plant origin with health benefit properties, such as anthocyanin is an important aim in plant genetic engineering. In this study, Malus domestica MYB10 (MdMYB10) gene, was used for Agrobacterium tumefaciens-mediated transformation of two SBS-02 and SBS-04 sugar beet lines. The impact of different light regimes on plant tissue culture from a combination of light, dark/light and dark was investigated. The results of this study showed that the MdMYB10 gene was successfully integrated into the selected purple transgenic lines, suggesting that the expression of MdMYB10 gene in sugar beet shoots can be used as a screenable markers for transformation, possibly replacing antibiotic resistant genes. Furthermore, the results of the antibacterial activity of transgenic plants extracts showed that the total extract obtained from transgenic lines significantly (P < 0.01) inhibited the growth and development of Enterococcus faecium and Enterococcus faecalis bacteria compared to the non-transgenic plants. The results of this study showed that the combination of betalain with vancomycin demonstrated a synergistic antimicrobial effect, also, suggesting that the expression of MdMYB10 may play a dual role by accumulating betalain and exhibiting a screenable markers function.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13205-022-03120-7.
