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Related Experiment Video

Updated: Oct 4, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Directed Evolution of Aptamer Discovery Technologies.

Diana Wu, Chelsea K L Gordon, John H Shin

Accounts of Chemical Research

|February 7, 2022

Summary

This summary is machine-generated.

Researchers developed four advanced techniques to accelerate the discovery of high-quality aptamers, enabling robust molecular recognition for challenging targets. These methods improve aptamer affinity and specificity for diverse biomedical applications.

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Last Updated: Oct 4, 2025

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Area of Science:

Biotechnology and Molecular Biology
Nucleic Acid Aptamer Technology
Diagnostic Reagent Development

Background:

Antibodies are widely used but have limitations in emerging applications.
Nucleic acid aptamers offer advantages over antibodies for specific applications.
Generating high-affinity and specific aptamers for biomedical use is challenging.

Purpose of the Study:

To describe four novel laboratory techniques for accelerating aptamer discovery.
To enable the generation of high-quality aptamers with robust binding capabilities.
To overcome challenges in identifying aptamers for difficult molecular targets.

Main Methods:

Particle display coupled with fluorescence-activated cell sorting (FACS) for affinity-based isolation.
Multiparametric particle display (MPPD) using two-color FACS for affinity and specificity screening.
Click chemistry-based particle display (click-PD) for generating and screening modified aptamers.
Non-natural aptamer array (N2A2) platform for high-throughput characterization of modified aptamers.

Main Results:

Particle display accelerates high-affinity aptamer isolation with reduced bias.
MPPD yields aptamers with excellent target binding in complex biological matrices.
Click-PD and N2A2 enable robust aptamers with modified bases for challenging targets.
Developed aptamers demonstrate enhanced affinity and specificity for target molecules.

Conclusions:

These advancements make aptamer technology more accessible, efficient, and robust.
The developed methods facilitate broader applications of aptamers in molecular recognition and detection.
Improved aptamer discovery accelerates progress in molecular biology and clinical diagnostics.