Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

26.6K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.6K
Protein-protein Interfaces02:04

Protein-protein Interfaces

13.9K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
13.9K
Protein Networks02:26

Protein Networks

4.1K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.1K
RNA-seq03:21

RNA-seq

10.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.5K
Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antigen-Pulsed Dendritic Cell-Derived Dexosomes Induce a Robust Th1-Biased Antigen-Specific Humoral and Cellular Immunity in Mice against Latent <i>Mycobacterium tuberculosis</i> Antigen <i>Rv2626c</i>.

ACS infectious diseases·2026
Same author

Unveiling the Healing Potential of Marsilea minuta Linn. (Sunishannaka): An Integrative Overview of Phytochemistry, Therapeutic Value, and Toxicological Aspects.

Chemistry & biodiversity·2026
Same author

Molecular Identification of <i>Mycobacterium bovis</i> in Human Pulmonary Tuberculosis: Insights from a Tertiary Care Hospital in Gujarat, India.

The Journal of the Association of Physicians of India·2026
Same author

Evaluation of menstrual-related symptoms and associated factors among women: A cross-sectional study from Chandauli district, Uttar Pradesh, India.

Women & health·2026
Same author

Combining OCT and OCT angiography improves differentiation of mild cognitive impairment and Alzheimer's disease.

Alzheimer's research & therapy·2026
Same author

Examination of influence of microfluidic flow and antibody orientation on biosensor performance: A case study with CA125 electrochemical biosensor.

Bioelectrochemistry (Amsterdam, Netherlands)·2026

Related Experiment Video

Updated: Oct 4, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.5K

Detecting RNA-RNA interactome.

Suman Singh1,2, Sharmishtha Shyamal1, Amaresh C Panda1

  • 1Institute of Life Sciences, Nalco Square, Bhubaneswar, India.

Wiley Interdisciplinary Reviews. RNA
|February 8, 2022
PubMed
Summary
This summary is machine-generated.

RNA-RNA interactions are crucial for gene regulation and cellular processes. Advanced sequencing technologies help identify these interactions, paving the way for understanding their role in health and disease.

Keywords:
RNA interactionsRNA sequencingRNA structuresplicingtranslation

More Related Videos

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.2K
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.3K

Related Experiment Videos

Last Updated: Oct 4, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.5K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.2K
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.3K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA molecules are increasingly recognized for their complex roles in gene regulation.
  • RNA-RNA interactions, both intramolecular and intermolecular, are vital for RNA biogenesis, structure, and function.
  • These interactions influence diverse RNA types, including mRNAs, microRNAs, tRNAs, and circRNAs.

Purpose of the Study:

  • To review the mechanisms and functions of RNA-RNA interactions in eukaryotes.
  • To highlight the impact of novel RNA molecules and their interactions on gene expression.
  • To discuss the evolution of methods for identifying RNA-RNA interactions.

Main Methods:

  • Review of established biochemical methods for RNA-RNA interaction identification.
  • Application of high-throughput RNA-sequencing technologies for global interactome analysis.
  • Discussion of current limitations and future directions in RNA-RNA interaction detection.

Main Results:

  • RNA-RNA interactions are fundamental to the biogenesis and function of various RNA molecules.
  • High-throughput sequencing has identified numerous intramolecular and intermolecular RNA-RNA interactions.
  • These interactions are critical for RNA structure, function, and gene expression regulation.

Conclusions:

  • Understanding RNA-RNA interactions is essential for comprehending eukaryotic gene regulation.
  • Future unbiased, quantitative high-throughput technologies are needed for comprehensive transcriptome-wide interactome analysis.
  • The study of RNA-RNA interactomes holds promise for revealing insights into human health and diseases.