Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Heliomycin decrease in the pool of purine- and pyrimidine-containing compounds in bacterial cells].

P N Korolev, V G Bulgakova, A N Polin

    Mikrobiologiia
    |March 1, 1978
    PubMed
    Summary

    Heliomycin hinders the creation of essential purine and pyrimidine compounds in Staphylococcus aureus and Escherichia coli. This suggests the antibiotic disrupts RNA synthesis or cellular energy metabolism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Secondary Metabolites from Marine Microorganisms. II. Marine Fungi and Their Habitats.

    Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]·2018
    Same author

    [Streptomycetes in the Light of the Concept of Multicellularity of Bacteria.]

    Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]·2018
    Same author

    [Secondary Metabolites from Marine Microorganisms. I. Secondary Metabolites from Marine Actinomycetes].

    Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]·2016
    Same author

    [On Biofilms of Streptomycetes. II. Use in Biotechnology].

    Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]·2016
    Same author

    [Streptomycetes Biofilms. I. Occurrence and Formation].

    Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]·2015
    Same author

    [Microbial secondary metabolites as potential reserve of pharmaceuticals].

    Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]·2014

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Purines and pyrimidines are fundamental building blocks for nucleic acids.
    • Bacterial growth phases (lag and exponential) exhibit distinct metabolic activities.
    • Understanding antibiotic mechanisms is crucial for combating microbial resistance.

    Purpose of the Study:

    • To investigate the effect of heliomycin on purine and pyrimidine metabolism in bacteria.
    • To determine if heliomycin impacts RNA synthesis or cellular energy pathways.

    Main Methods:

    • Culturing Staphylococcus aureus and Escherichia coli.
    • Measuring the formation of purine and pyrimidine compounds.
    • Assessing cell membrane permeability.
    • Analyzing acid-soluble fractions of bacterial cells.

    Related Experiment Videos

    Main Results:

    • Heliomycin significantly inhibited the formation of purine and pyrimidine compounds in both bacterial species.
    • The observed decrease in these compounds was not due to altered membrane permeability.
    • Inhibition occurred during both lag and exponential growth phases.

    Conclusions:

    • Heliomycin interferes with the regulation of RNA synthesis in bacteria.
    • The antibiotic may exert its effect by influencing cellular energy metabolism.
    • Further research is warranted to elucidate the precise molecular targets of heliomycin.