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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Updated: Oct 4, 2025

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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Published on: April 8, 2017

High-throughput methods for genome editing: the more the better.

Yong Huang¹, Meiqi Shang¹, Tingting Liu¹

¹State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China.

Plant Physiology

|February 8, 2022

Summary

High-throughput genome editing using CRISPR/Cas technologies offers powerful tools for deciphering gene function and engineering traits in animals and plants. This review covers advances in CRISPR/Cas applications for genetic modification and future perspectives.

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Last Updated: Oct 4, 2025

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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Published on: April 8, 2017

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Area of Science:

Genetics and Genomics
Biotechnology
Molecular Biology

Background:

Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) technologies have revolutionized genome targeting.
CRISPR/Cas systems, coupled with high-throughput techniques, are now key strategies for genetic engineering in diverse organisms.

Purpose of the Study:

To review recent advancements in high-throughput genome-editing technologies in animals and plants.
To highlight key applications and future perspectives of CRISPR/Cas in biological systems.

Main Methods:

High-throughput design of targeted guide RNA (gRNA).
Construction of large-scale pooled gRNA and genome-editing libraries.
High-throughput detection and supervision of genome-editing events and products.

Main Results:

CRISPR/Cas systems enable efficient genome modification for trait engineering.
High-throughput approaches accelerate the application of genome editing in animals and plants.
Advances include gRNA design, library construction, and editing event detection.

Conclusions:

High-throughput genome editing holds significant promise for fundamental research and applied biotechnology.
Future applications span gene therapy, crop improvement, and broader biological engineering.