Do it yourself! - Initial experiences with self-synthesized CsTFA for RNA-SIP analyses
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Updated: Oct 4, 2025

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
Published on: May 31, 2024
Severin Weis1, Susanne Jacksch1, Marius Welkerling1
1Microbiology and Hygiene Group, Institute of Precision Medicine, Faculty of Medical & Life Sciences, Furtwangen University, Villingen-Schwenningen, Germany.
Abstract:
Cesium trifluoroacetate (CsTFA) is a gradient medium for isopycnic centrifugation in RNA-based Stable Isotope Probing (RNA-SIP), an important means to link the structure and function of microbial communities. We report a protocol to easily synthesize CsTFA from cesium carbonate (Cs2CO3) and trifluoroacetic acid (TFA) and show that self-synthesized CsTFA performs similarly to commercial CsTFA in the separation of isotopically labelled and unlabelled bacterial RNA.
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