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Updated: Jan 22, 2026

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
Published on: June 28, 2019
Dataset on the analysis of β-galactosidase immobilization efficiency on aminomethyl polystyrene (AMP) resin in
Tabea Boehme1, Bernadette Straub1, Ursula Eschenhagen1
1Institute of Precision Medicine, Organic and Bioorganic Chemistry Labs, Faculty Health Medical and Life Sciences, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, Germany.
Abstract:
β-Galactosidase was covalently immobilized on AMP resin using a p-phenylenediisothiocyanate (PDC) linker. Resin loading was determined by measuring the protein concentration difference between the original lactase solution and the reactor supernatant using a Bradford assay. Enzyme activity of the supernatants was assessed with ONPG as the substrate, and lactose turnover rates were measured using a lactose solution. The dataset provides detailed results for five different approaches with several syringe reactors and two column reactors, enabling comparison of immobilization efficiency and catalytic performance. The dataset may be valuable for industrial applications where stable and efficient immobilized β-galactosidase systems are required, such as lactose-free dairy production.
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