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Related Experiment Videos

A simplified functional assay for protein C in plasma samples.

W Thiel, K T Preissner, U Delvos

    Blut
    |March 1, 1986
    PubMed
    Summary

    A new functional protein C (PC) assay accurately identifies PC deficiency, crucial for managing thromboembolic events. This simplified test uses readily available reagents and is suitable for routine clinical use.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Clinical Diagnostics

    Background:

    • Protein C (PC) plays a vital role in regulating hemostasis.
    • PC deficiency is linked to severe thromboembolic events, necessitating reliable diagnostic methods.
    • Existing diagnostic approaches may lack efficiency or require complex procedures.

    Purpose of the Study:

    • To develop and validate a novel, efficient functional assay for assessing protein C activity in plasma.
    • To establish a reliable method for early identification of protein C deficiency.
    • To simplify the diagnostic process for protein C-related coagulation disorders.

    Main Methods:

    • A new assay utilizing the thrombin/thrombomodulin complex for rapid activated PC generation.
    • Employing a chromogenic substrate to quantify the proteolytic capacity of activated PC.
    • Using rabbit lung homogenate as a substitute for purified thrombomodulin.

    Main Results:

    • The assay demonstrated specificity and accuracy, unaffected by common proteases like plasmin, streptokinase, and urokinase.
    • Heparin's inhibitory effect on PC activation was effectively neutralized by protamine sulfate.
    • Unselected patients (n=50) showed normal PC activity (100 +/- 12%), while phenprocoumon-treated patients (n=103) exhibited reduced PC activity (40 +/- 11%) and antigen levels (43 +/- 10%).

    Conclusions:

    • The developed functional protein C assay is specific, sensitive, and time-efficient.
    • This assay simplifies testing procedures without compromising accuracy, making it suitable for routine diagnostics.
    • The assay aids in the early and reliable identification of protein C deficiency and monitoring of anticoagulant therapy.

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