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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
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CRISPR-Cas system in microbial hosts for terpenoid production
1Faculty of Biotechnology, Chemistry and Environmental Engineering, Phenikaa University, Hanoi, Viet Nam.
Critical Reviews in Biotechnology
|February 10, 2022
Summary
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system offers an efficient way to engineer microbes for sustainable terpenoid production. This review highlights CRISPR-Cas applications in hosts like E. coli and S. cerevisiae for enhanced yields.
Area of Science:
- Metabolic Engineering
- Synthetic Biology
- Microbial Biotechnology
Background:
- Terpenoids are a vast class of natural products with diverse applications in pharmaceuticals, cosmetics, and biofuels.
- Microbial production offers a sustainable alternative to traditional extraction methods for terpenoids.
- Genome-editing technologies are crucial for optimizing microbial strains for enhanced metabolite production.
Purpose of the Study:
- To review the application of the CRISPR-Cas system in engineering microbial hosts for terpenoid production.
- To discuss the use of CRISPR-Cas variants like CRISPRi and CRISPRa for metabolic flux control.
- To explore future prospects of CRISPR-Cas technology in terpenoid biosynthesis.
Main Methods:
- Review of literature on CRISPR-Cas system applications in microbial terpenoid production.
- Focus on CRISPR-Cas9, CRISPRi (dCas9-based interference), and CRISPRa (dCas9-based activation).
- Analysis of combinatorial and individual system applications in key microbial hosts.
Main Results:
- The CRISPR-Cas system has been successfully applied to engineer major microbial hosts including Escherichia coli, Saccharomyces cerevisiae, Corynebacterium glutamicum, and Pseudomonas putida.
- CRISPRi and CRISPRa systems, individually or in combination, effectively control metabolic pathways to enhance terpenoid yields.
- Demonstrated efficiency of CRISPR-based tools in optimizing microbial cell factories for terpenoid biosynthesis.
Conclusions:
- The CRISPR-Cas system is a powerful and versatile tool for advancing microbial production of terpenoids.
- Continued development and application of CRISPR technologies promise further improvements in yield and sustainability.
- This review provides insights into the current landscape and future potential of CRISPR-mediated terpenoid engineering.
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