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Updated: Oct 4, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

Versioning biological cells for trustworthy cell engineering.

Jonathan Tellechea-Luzardo¹, Leanne Hobbs¹, Elena Velázquez²

¹Interdisciplinary Computing and Complex Biosystems (ICOS) Research Group, Newcastle University, Newcastle Upon Tyne, NE4 5TG, UK.

Nature Communications

|February 10, 2022

Summary

This summary is machine-generated.

A new cloud-based system provides version control for biotechnology, linking digital data to living cell samples. This enhances traceability and trustworthiness in engineering biology, making research more open and reproducible.

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Area of Science:

Biotechnology
Synthetic Biology
Bioinformatics

Background:

Full-stack biotechnology platforms for cell line reprogramming are emerging.
Advances in gene synthesis, editing, and integration with informatics drive this progress.
Traceability and trustworthiness are crucial for engineered cell lines and processes.

Purpose of the Study:

To develop a cloud-based version control system for biotechnology.
To organize digital data from cell engineering and link it to living samples.
To enhance transparency and reproducibility in engineering biology.

Main Methods:

Implementation of a cloud-based version control system.
Development of barcoding protocols for molecular linking.
Application to six species, including bacteria and eukaryote cells.

Main Results:

The system effectively tracks and organizes digital data from cell engineering.
Molecular links were established between digital data and living cell samples.
Protocols were demonstrated across diverse species.

Conclusions:

Version control is a significant step toward open and reproducible engineering biology.
The system improves traceability, sharing, and trustworthiness of engineered cell lines.
This approach supports the acceleration of biological product development.