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Updated: Oct 4, 2025

Upright Imaging of Drosophila Egg Chambers
Published on: March 13, 2015
Mapping Asymmetry in Collective Cell Migration: Lessons from Border Cells in Drosophila Oogenesis
1Department of Biological Sciences, Indian Institute of Science Education and Research-Kolkata, Nadia, West Bengal, India.
Abstract:
Asymmetry in the migrating group of cells is critical for efficient directed movement observed in normal development and in pathological conditions like tumor cell metastasis. This is conspicuously detected at the level of polarized protrusions and differential localization of various polarity proteins in collectively moving clusters. Over the years, border cell migration in Drosophila oogenesis has emerged as an excellent model system for studying polarity in the migrating group of cells. Here we report two protocols employing live cell imaging and tissue immunohistochemistry to evaluate the polarity in migrating border cell clusters.
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