Selective isolation and sensitive detection of lysozyme using aptamer based magnetic adsorbent and a new quartz
Gulay Bayramoglu1, Murat Kilic2, M Yakup Arica2
1Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Teknikokullar, Ankara, Turkey; Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Teknikokullar, Ankara, Turkey.
Abstract:
Magnetic chitosan beads and quartz crystal microbalance chip were decorated with lysozyme specific aptamer for isolation and detection of lysozyme, respectively. The lysozyme specific aptamer was immobilized on poly (dopamine) coated magnetic chitosan beads and the chip via Schiff base reaction. The percentage of the removal efficiency and purity of the isolated lysozyme from egg white were 87.6% and 91.8%, respectively. Further, the sensor system was contacted with different concentrations of lysozyme and other test proteins. This sensor system provided a method for the label-free, concentration-dependent, and selective detection of lysozyme with an observed detection limit of 17.9 ± 0.6 ng/mL. The sensor system was very selective and not significantly responded to the other tested proteins such as ovalbumin, trypsin, cytochrome C, and glucose oxidase. The prepared new sensor system showed a good durability and a high sensitivity for determination of lysozyme from solutions and whole egg white.
