Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

8.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
8.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

15.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
15.8K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

397
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
397
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

7.4K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Volumetric Single-Molecule Tracking Inside Subcellular Structures.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Maximising imaging volumes of expanded tissues for inverted fluorescence microscopy.

Biomedical optics express·2026
Same author

Multispectral live-cell imaging with uncompromised spatiotemporal resolution.

Nature photonics·2025
Same author

Increasing the acquisition speed in oblique plane microscopy via aliasing.

Biomedical optics express·2025
Same author

Increasing the acquisition speed in oblique plane microscopy via Aliasing.

bioRxiv : the preprint server for biology·2025
Same author

Real-time multi-angle projection imaging of biological dynamics.

Nature methods·2021

Related Experiment Video

Updated: Oct 3, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.5K

Answering some questions about structured illumination microscopy.

James D Manton1

  • 1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|February 14, 2022
PubMed
Summary

Structured illumination microscopy (SIM) enhances resolution beyond conventional methods. Achieving true super-resolution requires a non-polynomial response to light, moving beyond current diffraction limits.

Keywords:
extended-resolutionfluorescencestructured illuminationsuper-resolution

More Related Videos

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

4.3K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.9K

Related Experiment Videos

Last Updated: Oct 3, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.5K
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

4.3K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.9K

Area of Science:

  • Optical Microscopy
  • Super-resolution Imaging
  • Biophysics

Background:

  • Structured illumination microscopy (SIM) offers enhanced resolution compared to wide-field microscopy.
  • SIM's capabilities have expanded since its 1999 inception, including live-cell imaging and integration with other techniques.
  • Despite advancements, the underlying principles of SIM are not widely understood.

Purpose of the Study:

  • To address key questions regarding SIM's principles and limitations.
  • To explain why conventional SIM remains diffraction-limited.
  • To propose pathways for achieving true diffraction-unlimited super-resolution with SIM.

Main Methods:

  • Review and synthesis of existing SIM knowledge.
  • Explanation of diffraction limits in SIM.
  • Derivation of a real-space reconstruction approach for SIM and image scanning microscopy (ISM).
  • Bibliometric analysis of SIM development.

Main Results:

  • Clarification of SIM's current diffraction-limited status.
  • Identification of the need for non-polynomial light response for true super-resolution.
  • Presentation of a versatile real-space reconstruction method applicable to various illumination patterns.
  • Analysis of SIM's technological evolution over 20 years.

Conclusions:

  • SIM's potential for super-resolution is significant but currently constrained.
  • Advancing SIM requires moving beyond nonlinear responses to non-polynomial ones.
  • New reconstruction algorithms enhance SIM and ISM data processing capabilities.
  • Future work may focus on optimizing light-pattern interactions for ultimate resolution.