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Published on: June 30, 2022
Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the
Adelaine Kwun-Wai Leung1, Yasushi Kondo2,3, Daniel A Pomeranz Krummel4
1Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Abstract:
Cryo-electron microscopy has greatly advanced our understanding of how the spliceosome cycles through different conformational states to conduct the chemical reactions that remove introns from pre-mRNA transcripts. The Cryo-EM structures were built upon decades of crystallographic studies of various spliceosomal RNA-protein complexes. In this review we give an overview of the crystal structures solved in the Nagai group, utilizing many of the strategies to design crystal packing as described in the accompanying paper.
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