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Light-Emitting Diode Laser Therapy for Hyperoxia-Induced Retinal Abnormalities
Maha Sabry Abd Eldaiem1, Salwa Abdelkawi Ahmed2, Aziza Abdelmonem Elsaeid1
1Physics Department, Faculty of Science, Al Azhar University (Girls), Cairo, Egypt.
Journal of Lasers in Medical Sciences
|February 14, 2022
Summary
Low-level laser therapy effectively mitigates retinal damage caused by hyperoxia. This treatment improves retinal structure and oxidative balance, offering a potential therapeutic approach for hyperoxia-induced eye injuries.
Area of Science:
- Ophthalmology
- Biomedical Engineering
- Cell Biology
Background:
- Hyperoxygenation can lead to retinal tissue damage through oxidative stress and apoptosis.
- Reactive oxygen species (ROS) play a key role in hyperoxia-induced cellular injury.
Purpose of the Study:
- To investigate the protective effects of low-level laser therapy (LLLT) on retinal damage induced by acute hyperoxia in an animal model.
- To evaluate biochemical and histological changes in the retina following hyperoxia and LLLT.
Main Methods:
- Wistar albino rats were exposed to 90% oxygen for up to two weeks.
- One subgroup received 670 nm light-emitting diode laser treatment.
- Retinal tissues were analyzed for protein content, SDS-PAGE, total antioxidant capacity (TAC), hydrogen peroxide (H2O2), malondialdehyde (MDA), and histology.
Main Results:
- Two weeks of hyperoxia increased retinal protein content, decreased TAC, and elevated H2O2 and MDA levels.
- Histological analysis showed photoreceptor fragmentation and neovascularization in hyperoxia-exposed retinas.
- LLLT treatment significantly improved retinal protein content, oxidative status, and histological structure.
Conclusions:
- Light-emitting diode laser therapy demonstrates efficacy in reducing hyperoxia-induced retinal damage.
- LLLT presents a promising therapeutic strategy for managing oxidative stress-related retinal conditions.
