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Nerve Decellularized Matrix Composite Scaffold With High Antibacterial Activity for Nerve Regeneration
Yan Kong1, Di Wang1, Qufu Wei1
1Key Laboratory of Eco-Textiles, Ministry of Education, College of Textile Science and Engineering, Jiangnan University, Wuxi, China.
Frontiers in Bioengineering and Biotechnology
|February 14, 2022
Summary
A novel nerve decellularized matrix-chitosan scaffold offers high biocompatibility and antimicrobial properties for peripheral nerve regeneration. This material effectively supports Schwann cell growth, showing promise for neural tissue engineering applications.
Area of Science:
- Biomaterials Science
- Neural Tissue Engineering
- Regenerative Medicine
Background:
- Nerve decellularized matrix (NDM) is a promising material for peripheral nerve regeneration due to its natural composition and structure.
- Existing NDMs often lack simultaneous biocompatibility, infection resistance, and effective nerve regeneration promotion.
- Chitosan (CS) possesses inherent antimicrobial properties and biocompatibility.
Purpose of the Study:
- To develop a porous nerve decellularized matrix-chitosan (NDM-CS) scaffold with enhanced antimicrobial activity and biocompatibility.
- To evaluate the scaffold's efficacy in supporting Schwann cell growth, proliferation, polarization, and migration.
- To assess the potential of the NDM-CS scaffold for peripheral nerve regeneration applications.
Main Methods:
- A one-step method was employed to combine NDM and chitosan (CS) to create the NDM-CS scaffold.
- The scaffold's porosity, hydrophilicity, biocompatibility, and antibacterial effects against *Escherichia coli* and *Staphylococcus aureus* were evaluated.
- The scaffold's ability to support Schwann cell viability, proliferation, polarization, and migration was assessed, with specific attention to the E2C1 group.
Main Results:
- The NDM-CS scaffold exhibited high porosity and hydrophilicity, facilitating cell growth and nutrient exchange.
- The scaffold demonstrated significant antibacterial activity against both *E. coli* and *S. aureus*, reducing infection risk.
- The E2C1 group of the NDM-CS scaffold showed the strongest enhancement of Schwann cell proliferation, polarization, and migration, indicating positive effects on nerve injury repair.
Conclusions:
- The developed NDM-CS scaffold successfully integrates the benefits of NDM and CS, offering high biocompatibility and potent antimicrobial activity.
- The scaffold effectively supports critical cellular functions of Schwann cells, essential for nerve regeneration.
- This NDM-CS scaffold presents a promising candidate for future applications in neural tissue engineering and peripheral nerve repair.
