Related Experiment Video
Updated: Oct 3, 2025

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
Published on: August 7, 2021
riboviz 2: a flexible and robust ribosome profiling data analysis and visualization workflow
Alexander L Cope1, Felicity Anderson2, John Favate1
1Department of Genetics, Rutgers University, Piscataway, NJ 08854-8082, USA.
Motivation:
Ribosome profiling, or Ribo-seq, is the state-of-the-art method for quantifying protein synthesis in living cells. Computational analysis of Ribo-seq data remains challenging due to the complexity of the procedure, as well as variations introduced for specific organisms or specialized analyses.
Results:
We present riboviz 2, an updated riboviz package, for the comprehensive transcript-centric analysis and visualization of Ribo-seq data. riboviz 2 includes an analysis workflow built on the Nextflow workflow management system for end-to-end processing of Ribo-seq data. riboviz 2 has been extensively tested on diverse species and library preparation strategies, including multiplexed samples. riboviz 2 is flexible and uses open, documented file formats, allowing users to integrate new analyses with the pipeline.
Availability And Implementation:
riboviz 2 is freely available at github.com/riboviz/riboviz.
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