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Updated: Oct 3, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Molecular characterization of the gut microbiome in egyptian patients with remitting relapsing multiple sclerosis
Jaidaa Mekky1, Richard Wani1, Sameh M Said1
1Department of Neuropsychiatry, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Background:
Multiple Sclerosis is the most common chronic demyelinating disease of the central nervous system, representing the main cause of non-traumatic disabling disorders in young adults. The etiology of Multiple Sclerosis is not fully appreciated, although strong evidence points to genetic and environmental factors. The role of the gut microbiome in Multiple Sclerosis pathogenesis is a rapidly emerging area of study in that field.
Aim:
The aim of the present study is to identify the gut microbiome that are likely related to Multiple Sclerosis as well as their possible role in the susceptibility and the course of the disease.
Methods:
Thirty Egyptian patients with relapsing remitting multiple sclerosis, who presented to the Multiple Sclerosis Clinic of Alexandria University Hospital were enrolled in the study. These were diagnosed according to the McDonald 2017 criteria. A cross matching control group of 20 healthy subjects of similar age and sex was included. Stool specimens were taken from each for detection of gut microbiome profile by quantitative SYBR Green Real Time PCR assay.
Results:
The present study demonstrate that RRMS patients have a distinct gut microbiome compared to healthy controls, with certain gut microbes showing decreased or increased abundance in Multiple Sclerosis patients compared to controls. Multiple Sclerosis patients have significantly higher B. fragilis than the normal control. Although the level of Prevotella, and Lactobacilli appear much less in MS patients than the control, the difference was not statistically significant. The same for C. perfringes which was higher in Multiple Sclerosis patients.
Conclusion:
The overall results of the study agree with previous studies reporting that patients with Multiple Sclerosis in Egypt exhibit microbial gut dysbiosis. However, the results of individual bacteria may differ, according to the age, treatment, level of physcial exercise and type of therapy at the time of enrollment.
