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AC-Electroosmosis-Assisted Surface Plasmon Resonance Sensing for Enhancing Protein Signals with a Simple Kretschmann

Kyohei Terao1, Shohei Kondo1

  • 1Department of Intelligent Mechanical Systems Engineering, Kagawa University, Takamatsu-shi 761-0396, Japan.

Sensors (Basel, Switzerland)
|February 15, 2022
PubMed
Summary

This study introduces a novel surface plasmon resonance (SPR) sensor chip using AC electroosmosis for enhanced protein binding. The new chip design significantly improves analyte detection by creating circulating flow, boosting binding efficiency.

Keywords:
AC electroosmosisprotein detectionsurface plasmon resonance sensing

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Surface Science

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical sensing technique widely used for detecting molecular interactions.
  • Enhancing analyte binding efficiency is crucial for improving the sensitivity of SPR sensors.
  • Conventional SPR chips often face limitations in mass transport and mixing, affecting binding kinetics.

Purpose of the Study:

  • To develop and evaluate a novel SPR sensor chip incorporating a comb-shaped microelectrode array.
  • To investigate the effect of AC electroosmosis-induced circulating flow on analyte binding.
  • To demonstrate improved protein detection sensitivity using the developed SPR sensor chip.

Main Methods:

  • Fabrication of SPR sensor chips with comb-shaped microelectrodes using standard UV lithography.
  • Implementation of AC electroosmosis by applying alternating current (AC) voltage to the microelectrodes.
  • Conducting sensing experiments using a Kretschmann-type SPR measurement system.
  • Evaluating protein binding enhancement by measuring immunoglobulin G (IgG) binding to immobilized anti-IgG antibodies.

Main Results:

  • The fabricated chip successfully induced circulating flow near the sensor surface via AC electroosmosis.
  • A significant enhancement in protein binding was observed compared to conventional SPR chips.
  • The amount of IgG binding increased by a factor of 1.7, indicating improved protein signal.

Conclusions:

  • The novel SPR sensor chip with AC electroosmosis effectively enhances analyte binding through induced circulating flow.
  • This technology offers a promising approach for improving the sensitivity and performance of SPR-based biosensing.
  • The comb-shaped microelectrode array design is a viable strategy for boosting molecular binding in SPR sensors.