Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

"Panning" for lymphocytes: a method for cell selection.

L J Wysocki, V L Sato

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The molecular evolution of the immune response.

    Immunology today·2014
    Same author

    Spontaneous autoimmunity in mice that carry an IghV partial transgene: a required arginine in VHCDR3.

    Lupus·2009
    Same author

    Predominance of a novel splenic B cell population in mice expressing a transgene that encodes multireactive antibodies: support for additional heterogeneity of the B cell compartment.

    International immunology·2001
    Same author

    A receptor presentation hypothesis for T cell help that recruits autoreactive B cells.

    Journal of immunology (Baltimore, Md. : 1950)·2001
    Same author

    Activation and anergy in bone marrow B cells of a novel immunoglobulin transgenic mouse that is both hapten specific and autoreactive.

    Immunity·2001
    Same author

    Sequence heterogeneity in Ig kappa transcripts from single B lymphocytes.

    Molecular immunology·2000
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Researchers created a simple method to separate T and B lymphocytes using antibody-coated plastic dishes. This technique effectively isolates B lymphocytes while preserving the function of both cell types for further study.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Fractionation of lymphocytes is crucial for immunological research.
    • Existing methods can be complex or compromise cell viability.

    Purpose of the Study:

    • To develop a straightforward and effective method for separating T and B lymphocytes.
    • To provide a technique that maintains the functional integrity of separated cell populations.

    Main Methods:

    • Utilizing plastic dishes coated with anti-immunoglobulin (Ig) antibodies.
    • Selective adherence of splenic B lymphocytes to the coated dishes.
    • Separation of adherent (B cells) and nonadherent (T cells) populations via gentle pipetting.

    Main Results:

    • High purity separation: 97% of adherent cells were Ig-positive (B lymphocytes), and 98% of nonadherent cells were Ig-negative (T lymphocytes).

    Related Experiment Videos

  • The method is sensitive, capable of isolating cell populations as small as 2% of the total.
  • Separated cell populations retained their immunological function.

    • Conclusions:

    • The anti-Ig coated plastic dish method offers a simple, efficient, and functional approach for T and B lymphocyte fractionation.
    • This technique is adaptable for selecting cells based on various surface antigens.
    • The method holds broad utility for immunological and cell separation studies.