Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

329
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
329
Coulometry: Overview01:00

Coulometry: Overview

1.7K
Coulometry is one of the rapid, most accurate, and precise analytical techniques that determine the quantity of an analyte by measuring the electrical charge needed for its complete electrolysis without using any analytical standards. The total charge passed during electrolysis correlates with the analyte amount by Faraday's laws of electrolysis. For accurate coulometric measurements, a charge equal to Faraday's constant multiplied by the number of electrons involved in the relevant...
1.7K
Controlled-Current Coulometry: Overview01:27

Controlled-Current Coulometry: Overview

350
Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
350
Controlled-Current Coulometry: Coulometric Titration01:18

Controlled-Current Coulometry: Coulometric Titration

262
Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...
262
Electrodes: Overview01:17

Electrodes: Overview

1.9K
 Electrochemical measurements are conducted in an electrochemical cell composed of various components that control and measure the current and potential. One fundamental component is electrodes, conductive materials that enable electron transfer reactions at their surfaces.
There are two main types of electrodes in electrochemical cells. The first type, known as the working or indicator electrode, has a potential that is sensitive to the analyte's concentration and reacts to changes in...
1.9K
Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

2.9K
A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Printable Potentiometric Ion-Selective Electrodes Based on Carbon Fiber and Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene Nanoflakes: Eliminating Complex Modifications.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Transfer of Sodium Ion across Interface between Na<sup>+</sup>-Selective Electrode Membrane and Aqueous Electrolyte Solution: Can We Use Nernst Equation If Current Flows through Electrode?

Membranes·2024
Same author

Memristive Effect in Ti<sub>3</sub> C<sub>2</sub> T<sub>x</sub> (MXene) Polyelectrolyte Multilayers.

Chemphyschem : a European journal of chemical physics and physical chemistry·2023
Same author

Evaluation of pharmacist impact on diabetes outcomes for telehealth and hybrid care delivery versus in-office visits.

Journal of the American Pharmacists Association : JAPhA·2023
Same author

Locus-Specific Bisulfate NGS Sequencing of GSTP1, RNF219, and KIAA1539 Genes in the Total Pool of Cell-Free and Cell-Surface-Bound DNA in Prostate Cancer: A Novel Approach for Prostate Cancer Diagnostics.

Cancers·2023
Same author

Voltammetric Ion Sensing with Ionophore-Based Ion-Selective Electrodes Containing Internal Aqueous Solution, Improving Lifetime of Sensors.

Membranes·2022

Related Experiment Video

Updated: Oct 3, 2025

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
08:03

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Published on: April 18, 2013

17.4K

Constant Potential Coulometric Measurements with Ca2+-Selective Electrode: Analysis Using Calibration Plot vs.

Anna Bondar1, Konstantin Mikhelson1

  • 1Chemistry Institute c/o, St. Petersburg State University, 26 Universitetsky Prospect, Stary Peterhof, 198504 St. Petersburg, Russia.

Sensors (Basel, Switzerland)
|February 15, 2022
PubMed
Summary

Charge curve fitting offers a new analytical method for calcium ion (Ca2+) analysis, comparable to calibration plots. An electronic capacitor speeds up measurements with ion-selective electrodes.

Keywords:
analysiscalcium ion activitycalibration plotcharge curve fittingconstant potential coulometry

More Related Videos

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

14.1K
Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation
08:41

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

25.1K

Related Experiment Videos

Last Updated: Oct 3, 2025

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
08:03

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Published on: April 18, 2013

17.4K
Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

14.1K
Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation
08:41

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

25.1K

Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Chemical Sensing

Background:

  • Traditional analysis of calcium ions (Ca2+) often relies on calibration plots.
  • Constant potential coulometric mode offers an alternative analytical approach.
  • Ion-selective electrodes are crucial tools for quantifying specific ions in solution.

Purpose of the Study:

  • To explore charge curve fitting as a novel analytical method in constant potential coulometric mode.
  • To compare the efficacy of charge curve fitting against traditional calibration plot analysis.
  • To investigate the impact of an electronic capacitor on measurement speed and accuracy.

Main Methods:

  • Utilized a calcium ion (Ca2+) selective electrode as a model system.
  • Performed analysis in pure calcium chloride (CaCl2) solutions.
  • Compared charge curve fitting with calibration plot analysis, with and without an electronic capacitor in series.

Main Results:

  • Both charge curve fitting and calibration plot methods yielded good results (within 2% error) when concentration differences were within three-fold.
  • Larger concentration variations led to increased errors (10-25%) for both techniques.
  • Incorporating an electronic capacitor significantly accelerated the electrode's response time.

Conclusions:

  • Charge curve fitting is a viable alternative to calibration plots for Ca2+ analysis in constant potential coulometric mode.
  • The accuracy of both methods is dependent on the ratio between initial and final concentrations.
  • Electronic capacitors enhance the practical application of ion-selective electrodes by improving response speed.