Fluorescent and Luminescent Methods to Detect Lipolysis.

Area of Science:

• Metabolic biochemistry
• Cellular physiology

Background:

• Intracellular lipolysis is key in adipocytes for energy release and thermogenesis.
• Fatty acid mobilization impacts thermogenic gene expression and systemic energy supply.
• Lipid storage and release occur in multiple tissues, necessitating broad measurement techniques.

Purpose of the Study:

• To present detailed protocols for measuring cellular lipolysis.
• To enable quantification of fatty acid and glycerol efflux.
• To offer a high-throughput method for intracellular fatty acid detection.

Main Methods:

• Enzymatic detection of fatty acid efflux using fluorescent assays.
• Enzymatic detection of glycerol efflux using colorimetric assays.
• Genetically encoded luminescent system for intracellular fatty acid measurement.

Main Results:

• Established protocols for enzymatic quantification of lipolysis.
• Developed a luminescent system for high-throughput fatty acid analysis.
• Demonstrated applicability across diverse cell types.

Conclusions:

• The described methods provide versatile tools for studying lipolysis.
• These assays facilitate research in adipocyte function and broader metabolic processes.
• High-throughput analysis of lipolysis is now more accessible.