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Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Stem Cell Therapy for Tissue Regeneration

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
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Related Experiment Video

Updated: Oct 3, 2025

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

Cryopreserved Stem Cells Incur Damages Due To Terrestrial Cosmic Rays Impairing Their Integrity Upon Long-Term

P Rocheteau¹, G Warot², M Chapellier²

¹Human Histopathology and Animal Models, Department of Infection & Epidemiology, Institut Pasteur, Paris, France.

Cell Transplantation

|February 16, 2022

Summary

Cryopreserved stem cells accumulate DNA double-strand breaks (DSBs) from natural radiation over time. Shielding cells from cosmic rays during long-term storage significantly reduced DSBs, preserving cell quality for future therapies.

Keywords:

hematopoietic stem cells mesenchymal stem cells muscle regeneration radiation stem cell biology

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Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Published on: January 25, 2019

Area of Science:

Regenerative Medicine
Radiation Biology
Cell Biology

Background:

Stem cells are crucial for tissue renewal and hold significant therapeutic potential.
Cryopreservation at liquid nitrogen temperatures is used for long-term stem cell storage.
Natural radiation exposure during storage can damage cryopreserved stem cells, potentially leading to DNA double-strand breaks (DSBs).

Purpose of the Study:

To investigate the impact of natural radiation on DNA integrity in cryopreserved stem cells over extended storage periods.
To assess the effect of shielding from cosmic rays on DSB accumulation in long-term cryostored stem cells.

Main Methods:

Cryopreservation of stem cells.
Long-term storage under different shielding conditions (including shielding from cosmic rays).
Quantification of DNA double-strand breaks (DSBs) in thawed cells.

Main Results:

Cryopreserved stem cells accumulate DNA damage, specifically DSBs, over time due to natural radiation.
Stem cells stored with complete shielding from cosmic rays exhibited significantly fewer DSBs compared to unshielded cells after long-term storage.
The accumulation of DSBs can impact cell viability and therapeutic efficacy upon thawing.

Conclusions:

Natural radiation, including cosmic rays, poses a risk to the DNA integrity of cryopreserved stem cells during long-term storage.
Shielding stem cells from cosmic radiation during cryostorage is a viable strategy to minimize DNA damage.
These findings have critical implications for optimizing cryostorage protocols and ensuring the quality control of stem cell banks for therapeutic applications.