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Updated: Oct 3, 2025
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Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
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Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
Pallavi Deolal1, Imlitoshi Jamir2, Krishnaveni Mishra1
1Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Nucleus (Austin, Tex.)
|February 16, 2022
Summary
A novel protein, Uip4p, is essential for maintaining nuclear shape and nuclear envelope integrity in yeast. Its regulation is crucial for proper nuclear pore complex distribution and overall cell function.
Area of Science:
- Cell Biology
- Molecular and Cellular Biology
- Yeast Genetics
Background:
- The nucleus, a defining organelle in eukaryotic cells, is enclosed by a double membrane bilayer perforated by nuclear pore complexes (NPCs).
- Nuclear morphology is stably maintained in yeasts, despite the absence of lamins, and shape changes are regulated.
- Understanding the factors regulating nuclear shape and function in Saccharomyces cerevisiae is crucial for cell biology research.
Purpose of the Study:
- To identify novel factors involved in the regulation of nuclear shape and function in Saccharomyces cerevisiae.
- To investigate the role of a newly identified protein, Uip4p, in nuclear morphology.
Main Methods:
- Utilized a fluorescence imaging-based approach to screen for proteins affecting nuclear shape and function.
- Performed localization studies to determine the cellular location of Uip4p.
- Analyzed the expression and localization patterns of Uip4p during cell growth.
Main Results:
- Identified a novel protein, Uip4p, essential for the regulation of nuclear morphology.
- Demonstrated that loss of Uip4p function compromises nuclear pore complex (NPC) function and nuclear envelope (NE) integrity.
- Showed that Uip4p localizes to the NE and endoplasmic reticulum (ER) network, with its localization and expression regulated during growth.
Conclusions:
- Uip4p plays a critical role in maintaining nuclear envelope integrity and nuclear pore complex function in Saccharomyces cerevisiae.
- The regulated localization and expression of Uip4p during growth are vital for proper NPC distribution.
- Uip4p represents a key factor in the regulation of nuclear shape and function in yeast.
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