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Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
Published on: November 25, 2017
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Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron
Mohit Jaiswal1, Trang T Tran1, Jiatong Guo1
1Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL 32611, USA. zguo@chem.ufl.edu.
The Analyst
|February 16, 2022
Abstract:
A novel method for spin labelling of sialoglycans on the cell surface is described. C9-Azido sialic acid was linked to glycans on live cells via CSTII-catalysed α2,3-sialylation utilizing azido-sialic acid nucleotide as a sialyl donor, which was followed by attachment of a spin label to the azide via click reaction. It enables the study of cell surface sialoglycans by EPR spectroscopy.

