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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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Updated: Oct 3, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Quantitative Translation Proteomics Using mePROD.

Kevin Klann1, Christian Münch2,3,4

  • 1Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2022
PubMed
Summary
This summary is machine-generated.

Multiplexed enhanced protein dynamic mass spectrometry (mePROD MS) offers robust, cost-efficient proteome-wide translation quantification. This method achieves high identification rates and temporal resolution of minutes, improving upon existing techniques.

Keywords:
Bottom-up proteomicsMass spectrometryProtein dynamicsProteomicsSILACTMTTranslationpSILAC

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Area of Science:

  • Proteomics
  • Molecular Biology
  • Biochemistry

Background:

  • Quantifying protein synthesis (translation) is crucial for understanding cellular processes.
  • Existing methods like TMT and pSILAC often require lengthy labeling periods.
  • Improving the efficiency and speed of proteome-wide translation analysis is needed.

Purpose of the Study:

  • To introduce Multiplexed enhanced protein dynamic mass spectrometry (mePROD MS) for robust protein translation quantification.
  • To demonstrate mePROD MS's ability to profile proteome-wide translatome changes with high temporal resolution.
  • To provide an easy and cost-efficient method for analyzing newly synthesized proteins.

Main Methods:

  • Combining tandem mass tags (TMT) with pulsed stable isotope labeling in cell culture (pSILAC).
  • Utilizing a carrier signal to boost survey scan intensity in mass spectrometry.
  • Implementing mePROD MS for enhanced peptide identification rates.

Main Results:

  • Achieved robust quantification of protein translation in cell culture.
  • Significantly increased identification rates of newly synthesized peptides.
  • Enabled temporal resolution of translatome changes down to minutes.

Conclusions:

  • mePROD MS provides an efficient and cost-effective approach for proteome-wide translation profiling.
  • The method overcomes limitations of long labeling times in previous techniques.
  • mePROD MS facilitates rapid analysis of dynamic changes in protein synthesis.