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Updated: Oct 3, 2025

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
Published on: October 11, 2022
Arrested crossover precursor structures form stable homologous bonds in a Tetrahymena meiotic mutant
Miao Tian1, Kazufumi Mochizuki2, Josef Loidl1
1Department of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna, Austria.
Abstract:
Meiotic DNA double-strand breaks produce reciprocally exchanged DNA strands, which mature into chiasmata that hold homologous chromosomes together as bivalents. These bivalents are subsequently separated in the first meiotic division. In a mutant lacking the newly identified Tetrahymena gene APRO1 (Anaphase promoting 1), meiosis is arrested by the end of prophase. Mature chiasmata are not formed but bivalents are connected via a molecular precursor structure. In-depth analysis of this arrested intermediate structure may help to elucidate the noncanonical molecular recombination pathway in Tetrahymena.
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