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Intracellular Mechanical Drugs Induce Cell-Cycle Altering and Cell Death.

María Isabel Arjona1, Marta Duch1, Alberto Hernández-Pinto2

  • 1Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola del Vallès, Barcelona, 08193, Spain.

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PubMed
Summary
This summary is machine-generated.

Intracellular mechanical cues, like nanodevices, significantly impact cell function and fate. Device shape and stiffness are key factors influencing cell-cycle progression and promoting cell death.

Keywords:
biomaterialscell cyclemechanobiologynanomaterialsnanomedicinesilicon chips

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Area of Science:

  • Materials Science
  • Cell Biology
  • Biophysics

Background:

  • Extracellular mechanical cues influence cell function and fate.
  • The impact of intracellular mechanical cues on cell mechanics is not well understood.

Purpose of the Study:

  • To investigate how intracellular mechanical cues affect cell mechanics, cell cycle, and cell death.
  • To determine the role of device properties (size, shape, stiffness) in mechanical impairment.
  • To explore the forces involved in intracellular confinement and cell division.

Main Methods:

  • Internalization of reproducible nanodevices with controlled size, shape, and stiffness into HeLa cells.
  • Observation of cell-cycle deviations and cell death induction.
  • Analysis of nanodevice effects on mitosis, spindle centering, and chromosome alignment.
  • Measurement of forces generated by the cell spindle.

Main Results:

  • Internalized nanodevices induce cell-cycle deviations and promote cell death.
  • Device shape and stiffness are primary determinants of mechanical impairment.
  • Nanodevices can prevent spindle centering and correct chromosome alignment during mitosis.
  • The cell spindle generates forces exceeding 114 nN, capable of overcoming intracellular confinement.

Conclusions:

  • Intracellular mechanical constraints play a critical role in defining cell function and fate.
  • Nanomedicine approaches can be used to study and manipulate intracellular mechanics.
  • This work provides a foundation for understanding the impact of intracellular mechanical forces on cellular processes.