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Updated: Oct 3, 2025
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
Published on: June 7, 2016
Correlation between Structural Transformations in Mesenteric Lymph Nodes and the Levels MicroRNA during
A V Kabakov1, O V Kazakov2, A F Poveshchenko2,3
1Research Institute of Clinical and Experimental Lymphology - Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. kabakov_av85@mail.ru.
Abstract:
We studied interrelationships between the cytoarchitectonics of the mesenteric lymph node and the levels of microRNA-21, microRNA-221/222, and microRNA-429 in the lymph, blood serum, and breast tissues in female Wistar rats with chemically induced breast cancer. After polychemotherapy, significant correlations were found between miRNA-221 and the number of lymphoblasts in the germinal centers and between miRNA-222 and the number of lymphoblasts in the germinal centers and macrophages in the medullary cords of the mesenteric lymph nodes.
Insights
This study explored links between breast cancer, lymph node structure, and specific microRNAs in rats. Post-treatment, microRNA-221 and microRNA-222 levels correlated with immune cell populations in mesenteric lymph nodes.
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- Breast cancer involves complex interactions between tumor cells and the immune system.
- MicroRNAs (miRNAs) play critical roles in cancer development and immune regulation.
- Mesenteric lymph nodes are key sites for immune responses to systemic conditions like cancer.
Purpose of the Study:
- To investigate the relationship between mesenteric lymph node cytoarchitectonics and specific miRNA levels in breast cancer.
- To analyze miRNA profiles in lymph, serum, and breast tissue following polychemotherapy.
- To identify correlations between miRNA expression and immune cell populations within lymph nodes.
Main Methods:
- Chemically induced breast cancer model in female Wistar rats.
- Analysis of microRNA-21, microRNA-221/222, and microRNA-429 levels in lymph, blood serum, and breast tissue.
- Histological examination of mesenteric lymph node cytoarchitectonics.
- Correlation analysis between miRNA levels and immune cell counts (lymphoblasts, macrophages).
Main Results:
- Significant correlations were observed between microRNA-221 and lymphoblasts in germinal centers.
- MicroRNA-222 levels correlated with lymphoblasts in germinal centers and macrophages in medullary cords.
- These findings emerged after polychemotherapy treatment.
Conclusions:
- Specific microRNAs (miRNA-221, miRNA-222) are associated with changes in immune cell composition within mesenteric lymph nodes during breast cancer treatment.
- These miRNAs may serve as potential biomarkers for monitoring immune responses in breast cancer.
- Further research is warranted to elucidate the functional roles of these miRNAs in cancer-associated lymph node alterations.
