Factors affecting the yield of viable cells of Corynebacterium pseudotuberculosis in a liquid medium

Growth of Corynebacterium pseudotuberculosis in an undefined liquid medium was studied with the aim of obtaining uniform, single-cell suspensions of the organism suitable for experimental infection. Adequate yields of viable cells were shown to be dependent on a fermentable substrate and a buffering system in a medium containing Tween 80. The development of Nutrient Tween 80 Broth for the culture of C. pseudotuberculosis is considered to be a significant advance particularly for studies involving animal inoculation.