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Updated: Oct 3, 2025
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Simple and programmed three-dimensional DNA tweezer for simultaneous one-step detection of ochratoxin A and
Ruipeng Chen1, Zefeng Mao1, Ran Lu1
1Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China.
Abstract:
Three-dimensional (TD) deoxyribonucleic acid (DNA) tweezers were programmed for one-step identification and detection of ochratoxin A (OTA) and zearalenone (ZEN). The unfolding of the TD-DNA tweezers by aptamers specific to these two mycotoxins "turned" the fluorescent signals "on." The bonding of the aptamers to their corresponding targets in OTA and ZEN "turned" the fluorescent signals and the DNA tweezers "off." The detection limit of the TD-DNA tweezers for OTA and ZEN was 0.032 and 0.037 ng mL-1, respectively. The feasibility of this method was tested using two samples. Detection via this method increased the recovery of OTA and ZEN from 95.8% to 110.2%. Spike recovery and certified food products were used to detect applicability in actual situations. Analyte detection in complex samples using TD-DNA tweezers is rapid, as the process involves a single operational step. This proposed design has considerable potential for application in mycotoxin detection.
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