Related Experiment Video
Updated: Oct 3, 2025
13:51
Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
10.0K
[A new pathophysiological element in severe asthma: GTPase Rac]
D Hassoun1, G Loirand2, V Sauzeau2
1Université de Nantes, CHU de Nantes, CNRS, Inserm, institut du thorax, 44000 Nantes, France.
Revue Des Maladies Respiratoires
|February 20, 2022
Summary
Rac GTPase signaling is crucial for severe asthma, impacting airway hyperresponsiveness and remodeling. Further research into its role in inflammation could lead to better treatments for uncontrolled asthma patients.
Area of Science:
- Pulmonology
- Molecular Biology
- Cell Signaling
Background:
- Asthma is a chronic airway disease characterized by hyperresponsiveness, bronchial remodeling, and inflammation.
- Severe asthma cases often remain uncontrolled despite advanced biotherapies.
- Understanding underlying signaling pathways is critical for addressing unmet needs in severe asthma management.
Purpose of the Study:
- To investigate the role of Rac GTPase signaling in the pathophysiological mechanisms of severe asthma.
- To evaluate the involvement of Rac GTPase in asthma-related inflammation.
Main Methods:
- This study focuses on evaluating the role of Rac GTPase in asthma.
- Specific methodologies are detailed within the full research paper.
Main Results:
- Rac GTPase has been linked to airway hyperresponsiveness and bronchial remodeling in asthma.
- Its specific role in asthma inflammation is currently under investigation.
Conclusions:
- Rac GTPase is a key molecular switch implicated in severe asthma.
- Further elucidation of its inflammatory functions may offer novel therapeutic targets for uncontrolled asthma.
Related Concept Videos
01:24Small GTPases - Ras and Rho
4.3K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
4.3K
02:14GTPases and their Regulation
8.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
8.9K
01:09GPCRs Regulate Adenylyl Cylase Activity
6.0K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.0K
01:22Activation and Inactivation of G Proteins
7.9K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
7.9K
02:38The Ras Gene
6.5K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
6.5K
01:26Asthma-II: Pathophysiology and Classification
3.2K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
3.2K