Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

443
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
443
RNA-seq03:21

RNA-seq

10.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.5K
RNA Editing02:23

RNA Editing

9.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.2K
Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K
Types of RNA01:20

Types of RNA

6.7K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Abiotic synthesis of RNase-resistant phosphodiester and pyrophosphate-linked polymers via thermodynamically controlled wet-dry cycling.

Scientific reports·2026
Same author

Exploratory inflammatory profiles in patients with depressive disorders and a history of cancer considering alcohol consumption.

General hospital psychiatry·2026
Same author

Argonaute and small RNAs in the nucleus: Mediators of gene silencing and activation.

Molecular therapy. Nucleic acids·2026
Same author

Translation-dependent degradation of cas12 mRNA triggered by an anti-CRISPR.

Nature·2026
Same author

Multivalent Peptide-DNA Conjugates Facilitate DNA Internalization in Cells Expressing Glycosaminoglycans.

ACS chemical biology·2026
Same author

A 2025 meeting report from the Venice lagoon: International graduate program <i>RNAmed - Future Leaders in RNA-based Medicine</i> meets on San Servolo.

RNA (New York, N.Y.)·2026

Related Experiment Video

Updated: Oct 3, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.9K

Innovative developments and emerging technologies in RNA therapeutics.

François Halloy1, Annabelle Biscans2, Katherine E Bujold3

  • 1Department of Paediatrics, Medical Sciences Division, University of Oxford, Oxford, UK.

RNA Biology
|February 21, 2022
PubMed
Summary

RNA therapeutics, including antisense oligonucleotides and small interfering RNAs, offer versatile gene targeting for disease treatment. Emerging RNA technologies show promise as biosensors and novel therapeutics, expanding drug design possibilities.

Keywords:
PROTACRNA-therapeuticsantisensedeliverynucleic acid technologiesoligonucleotide based artificial ribonucleasessaRNAsiRNAviral RNA

More Related Videos

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.5K
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
12:03

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

8.0K

Related Experiment Videos

Last Updated: Oct 3, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.9K
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.5K
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
12:03

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

8.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Discovery

Background:

  • RNA-based therapeutics represent a rapidly advancing field with significant potential for treating diverse diseases.
  • Antisense oligonucleotides and small interfering RNAs are established nucleic acid therapeutics targeting gene expression.
  • Recent advancements include messenger RNA vaccines and novel RNA modalities.

Purpose of the Study:

  • To provide a comprehensive overview of current RNA-based therapeutic technologies.
  • To highlight the potential of RNA as biosensors and therapeutics.
  • To discuss emerging RNA modalities and future directions in the field.

Main Methods:

  • Review of established and emerging RNA-based technologies.
  • Analysis of mechanisms of action and disease applications.
  • Exploration of novel RNA architectures and modalities.

Main Results:

  • RNA therapeutics offer versatile gene silencing, splicing modulation, and microRNA binding.
  • Messenger RNA technology has led to approved vaccines.
  • Innovative approaches like RNA minimal architectures and viral RNA show therapeutic promise.

Conclusions:

  • RNA-based therapeutics are a prominent tool in drug design and development.
  • Further research into novel RNA modalities will expand therapeutic applications.
  • RNA technologies hold significant potential for future medical treatments.