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Updated: Oct 3, 2025

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Published on: September 30, 2016
Engineering Destabilizing N-Termini in Plastids
Lioba Inken Winckler1,2,3, Nico Dissmeyer4,5,6
1Protein Metabolism Lab, Department of Plant Physiology, University of Osnabruck, Osnabruck, Germany.
Abstract:
Studying the stability of a protein dependent on its N-terminal residue requires a mechanism, which selectively exposes the amino acid at the N-terminus. Here, we describe the use of the tobacco etch virus (TEV) protease to generate a specific N-terminal amino acid in the stroma of the chloroplast. The established molecular reporter system further allows the quantification of the reporter protein half-life dependent on the identity of the N-terminal residue.
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