Related Experiment Video
Updated: Oct 2, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Osteometric reassociation of commingled human remains from a modern Greek sample using bone elements of the
Vasiliki Louka1, Ioanna Anastopoulou2, Konstantinos Moraitis2
1University of Leicester, School of Archaeology and Ancient History, University Rd, Leicester LE1 7RH, United Kingdom.
Abstract:
There is a high demand of osteometric methods to address the issue of commingled remains, however, the research in reassociating the crania with C1, and C1 with C2, as part of the craniovertebral junction, is limited. Reassociating crania with the postcranial skeleton can offer beneficial information for personal identification, as the cranium is broadly utilized for sex and age estimation. Moreover, cranium contributes to the facial reconstruction, a useful supplementary method for forensic identification. For this study, measurements were collected from 159 crania and 182 C1 and C2 vertebrae from the Athens Collection, representing adult males and females between 18 and 99 years. A reassociation method was produced utilizing osteometrics of articular surfaces. A supplementary sample of ten individuals was utilized as a test sample, originating from Athens Medical School. Seven equations were generated by simple linear regression analysis for the reassociation of the cranium to C1, and C1 to C2. The blind test results demonstrated that this method can be applied with 80-100% success. Overall, the results indicated that the produced regression models are a useful addition to the existing sorting methodologies, as they can be applied in mixed-sex and/or fragmented commingled osteological assemblages.
Related Concept Videos
Sutures of the Skull
Sutures are immobile joints between adjacent bones of the skull. The narrow gap between the bones is filled with dense, fibrous connective tissue that unites the bones. The long sutures located between the skull bones are not straight but instead follow irregular, tightly twisting paths. These twisting lines tightly...
Cranial Bones: Lateral View
The temporal bone forms the lower lateral side of the skull. The temporal bone is subdivided into several regions. The flattened upper portion is the squamous portion of the temporal bone. Below this area and projecting anteriorly is the zygomatic process of the temporal bone, which forms the posterior portion of the zygomatic arch. Posteriorly is the mastoid portion of the temporal bone. Projecting...
Cranial Bones: Superior and Posterior View
The frontal bone is the single bone that forms the forehead. At its anterior midline, between the eyebrows, there is a slight depression called the glabella. The frontal bone also forms the supraorbital margin of the orbit. Near the middle of this margin is the supraorbital foramen, the opening that provides passage for a sensory nerve to the forehead. The frontal bone is thickened just above each supraorbital margin,...
Gross Anatomy of Bone
The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The walls of the diaphysis are composed of dense and hard compact bone made of numerous osteons — the functional unit of the compact bone. The hollow region in the diaphysis is called the medullary cavity, which harbors the bone marrow. In infants and children, this marrow cavity is filled with red marrow, whereas in...
Classification of Bones
Long and Short Bones
The appendicular skeleton, particularly the upper and lower limbs, is primarily made of long and short bones. The...
Overview of the Skull
The cranial vault surrounds and protects the brain and houses the middle and inner ear structures. This cavity is bounded superiorly by the rounded top of the skull, which...

