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Updated: Aug 19, 2026

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
Published on: October 21, 2016
Glycidyl methacrylate copolymers as suitable supports for immobilizing antigens and antibodies
Abstract:
Glycidyl methacrylate copolymers modified with 1,6-diaminohexane were activated with glutaraldehyde. Such activated supports were directly bound to the chosen antisera. Prepared immunosorbents contained ca. 40 mg of bound protein and were able to purify 2-5 mg of antigen (alkaline proteinases Esperase and Maxatase) per 1 g of solid support. Chaotropic agent (2.5 M ammonium thiocyanate in phosphate or Tris buffer) enabled antigen to be released quickly, the properties of the immunosorbent remaining unchanged.
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