Experience with an homogeneous immunoassay for paracetamol (acetaminophen)

The performance of an homogeneous enzyme immunoassay (EMIT) for paracetamol (acetaminophen) as configured for the aca series of discretionary analysers (Du Pont de Nemours) was evaluated. The method as initially formulated by the manufacturers gave unacceptable performance which was improved upon, by reagent reformulation and improved kit manufacture procedures, by the manufacturer. The method was fast, precise and free from interference from a range of metabolites, drugs and endogenous physiological compounds. There was good correlation between this method and an enzymatic method for paracetamol (acetaminophen).