Related Experiment Video
Updated: Oct 2, 2025
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
Published on: July 7, 2022
Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse
Ferdinand Althammer1, Eric G Krause2,3,4, Anette D de Kloet5,3,4
1Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA 30302-5030, USA.
Abstract:
Here, we present a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, applied to the central amygdala oxytocin receptor-expressing astrocytes. This includes RNAse-free perfusion, combination of RNAscope and immunohistochemistry, and confocal imaging. This protocol provides detailed information about tissue handling and a comprehensive description of the RNAScope technique to label rat and mouse oxytocin receptor mRNA. We also describe three-dimensional reconstruction that allows the assessment of more than 70 different cellular parameters, powerful for studying astrocyte morphology and astrocyte-astrocyte interactions. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021) and Althammer et al. (2020).
