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A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
Xiaoqian Tang1,2,3,4,5,6, Qi Zhang1,2,3,4,5, Maria Isabel Pividori7
1Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
Biosensors
|February 24, 2022
Summary
A new aptamer-based lateral flow assay (LFA) offers a rapid and sensitive method for detecting patulin, a harmful mycotoxin in foods. This tool aids in routine food safety screening, ensuring healthier products for consumers.
Area of Science:
- Food Science
- Analytical Chemistry
- Biotechnology
Background:
- Patulin is a significant mycotoxin found in fruits and vegetables, posing risks to food safety and human health.
- Rapid and simple detection methods for patulin are crucial for routine screening and food surveys.
Purpose of the Study:
- To develop a sensitive aptamer-based lateral flow assay (FLA) for patulin detection.
- To utilize Streptavidin-functionalized gold nanoparticles for enhanced sensitivity.
Main Methods:
- Development of an aptamer-based lateral flow assay (FLA).
- Functionalization of gold nanoparticles with Streptavidin.
- Testing the assay's dynamic range, specificity, and limit of detection for patulin.
Main Results:
- The FLA demonstrated a wide dynamic range for patulin detection (2.7–139.8 ng/mL in buffer, 7.07–359.5 ng/mL in sample).
- The assay showed no cross-reactivity with other common mycotoxins (ZEN, OTA, AFB1, citrinin, TEA).
- The limit of detection was as low as 0.19 ng/mL (buffer) and 0.36 ng/mL (sample), with good recovery rates (83.3–107.1%) and low RSD (6.5–7.5%).
Conclusions:
- The developed aptamer-based FLA is a sensitive, rapid, and simple tool for on-site patulin determination.
- This method is suitable for routine screening of patulin in food products like apple juice.
- The assay's specificity and sensitivity contribute to improved food safety monitoring.
