Haemoprocessor: A Portable Platform Using Rapid Acoustically Driven Plasma Separation Validated by Infrared
Kamal Prakash Prasanna Ravindran Nair1,2,3, Thulya Chakkumpulakkal Puthan Veettil4, Bayden R Wood4
1Department of Mechanical & Aerospace Engineering, Monash University, Melbourne, VIC 3800, Australia.
This study introduces the Haemoprocessor, a portable microfluidic device using Travelling Surface Acoustic Waves (TSAW) for rapid plasma separation. It maintains chemical integrity, offering a reliable alternative to traditional methods for frequent diagnostic testing.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Microfluidics
Background:
- Plasma separation is crucial for diagnostics but faces logistical challenges.
- Existing microfluidic devices lack validation of sample chemical integrity.
- Frequent, rapid testing strains healthcare systems.
Purpose of the Study:
- To present the Haemoprocessor, a novel portable system for rapid plasma separation.
- To validate the chemical integrity of plasma separated using the Haemoprocessor.
- To compare the Haemoprocessor's performance against centrifugation.
Main Methods:
- Utilized Travelling Surface Acoustic Waves (TSAW) with overtones for plasma separation.
- Separated plasma from 20× diluted human blood in 2 minutes.
- Validated sample integrity using Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy and Principal Component Analysis (PCA).
Main Results:
- Achieved 98% red blood cell (RBC) removal.
- PCA demonstrated 92.21% separation between RBCs and plasma components.
- ATR-FTIR and PCA confirmed chemical integrity comparable to centrifugation.
Conclusions:
- The Haemoprocessor is a portable, robust, and open-fluidic system for efficient plasma separation.
- It offers validated chemical integrity, outperforming contemporary acoustic devices.
- The system shows potential as an effective platform for cell enrichment and plasma separation.
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