Related Experiment Video
Updated: Oct 2, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Understanding and correcting wavenumber error in interference pattern structured illumination imaging
Abstract:
The impacts of uncertainty in mirror movements in mechanically scanned interference pattern structured illumination imaging (IPSII) are discussed. It is shown that uncertainty in IPSII mirror movements causes errors in both the phase and amplitude of the Fourier transform of the resulting imaging. Finally, we demonstrate that iterative phase retrieval algorithms can improve the quality of IPSII images by correcting the phase errors caused by mirror movement uncertainties.
More Related Videos
11:47Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments
Published on: February 27, 2013
11:57Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
Published on: December 1, 2016
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Interference: Path Lengths
Two special sources may be considered when they are in phase. This can be easily achieved by feeding the two sources from the same source. An example would be synchronizing the two speakers by feeding them with the same source, such as the sound waves produced by a tuning fork. This setup ensures that the two sources have the same frequency and are...
NMR Spectrometers: Resolution and Error Correction